Tag
Part:BBa_K918000:Design
Designed by: steven denham Group: iGEM12_Colorado_State (2012-09-23)
Mating Factor Alpha Secretion Tag
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 29
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 29
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 29
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 29
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was taken from NCBI databases. We successfully synthesized it through Gibson Assembly and extracted it from yeast genomic DNA by PCR.
Source
Saccharomyces cerevisiae genome