Tag

Part:BBa_K918000:Design

Designed by: steven denham   Group: iGEM12_Colorado_State   (2012-09-23)


Mating Factor Alpha Secretion Tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 29
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 29
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 29
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 29
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was taken from NCBI databases. We successfully synthesized it through Gibson Assembly and extracted it from yeast genomic DNA by PCR.


Source

Saccharomyces cerevisiae genome

References